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產(chǎn)品中心免疫學(xué)抗體A00186THE™ His Tag Antibody, mAb, Mouse
產(chǎn)品型號(hào)：A00186
更新時(shí)間：2025-05-23
廠商性質(zhì)：代理商
訪 問(wèn) 量 ：3050
0592-5771844
產(chǎn)品分類
THE™ 代表了"THE unmistakable, THE irreplaceable, THE best quality"。金斯瑞選擇穩(wěn)定、高產(chǎn)的雜交瘤細(xì)胞克隆，然后使用相應(yīng)的抗原進(jìn)行篩選分離，制備出特異性高、親和力強(qiáng)的THE™ 系列金牌抗體。
您在實(shí)驗(yàn)中是否曾經(jīng)因?yàn)闃悠妨啃 ?biāo)簽只有部分暴露、融合蛋白變性或其它棘手的問(wèn)題而感到煩惱？金斯瑞THE™ 系列金牌抗體，讓您的這些煩惱迎刃而解！
同時(shí)，我們也為您提供其它優(yōu)質(zhì)的免疫學(xué)相關(guān)產(chǎn)品。如憑借**的多肽合成能力，我們能夠提供大多數(shù)抗體的封閉肽，讓您確認(rèn)觀察到的信號(hào)是由抗體與靶抗原之間的相互作用產(chǎn)生，而不是由于非特異性結(jié)合而產(chǎn)生。
靈敏度—比市場(chǎng)上其他暢銷的抗體更靈敏；
特異性—無(wú)交叉反應(yīng)；
親和力—-低解離常數(shù)Kd；
識(shí)別能力—能夠識(shí)別并結(jié)合廣泛的物種及表達(dá)系統(tǒng)的靶抗原；
低背景—無(wú)其它反應(yīng)背景；
便捷—適用范圍廣，產(chǎn)品全面；
穩(wěn)定—-好的批次間一致性，凍干狀態(tài)下，可至少穩(wěn)定保存2年。
Description | Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells. However, since 6XHis-tag is poorly immunogenic, it needs to be conjugated to KLH or some other carrier as an immunogen. After hundreds of selection cycles, researchers at GenScript successfully isolated an antibody against His-tag. THE™ His Tag Antibody, mAb, Mouse (subtype IgG1) has very high affinity. Tests performed at GenScript show that the antibody can also recognize 4xHis- and 5xHis-tags. This means that even if the 6xHis-tag is only partially exposed, it will still be recognized and bound by this antibody. THETM His Tag mAb is produced from mice ascites and purified by protein A affinity column. This antibody recognizes native as well as denatured forms of synthetic polyhistidine and polyhistidine-tagged fusion proteins. The product reacts with fusion proteins expressed in bacteria, insect cells, and mammalian cells. THETM His Tag mAb recognizes His tags placed at N-terminal, C-terminal, and internal regions of fusion proteins. THETM His Tag mAb can be used in Western blot analyses, Dot blot analyses, ELISA, immunofluorescent staining, and flow cytometry of cultured cells. |
| Host Species | Mouse |
| Conjugation | Unconjugated |
| Immunogen | A synthetic peptide HHHHHH coupled to KLH |
| Fusion Partner | Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells |
| Purification | Protein A affinity column |
| Subclass | IgG1, k |
| Clone ID | 6G2A9 |
| Concentration | 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
| Reconstitution | Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml. |
| Specificity | THE™ His Tag Antibody, mAb, Mouse recognizes C-terminal, N-terminal, and internal His tagged fusion proteins. |
| Storage | The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
| Note | GenScript can offer this product according to your requirement, including product size, buffer components,etc. |
| Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. ELISA: 0.05-0.2 µg/ml Western Blot: 0.1-0.2 µg/ml Immunoprecipitation (IP): 1 µg/ml Immunofluorescent staining: 1 µg/ml Flow cytometry (FACS): 1 µg/ml TR-FRET assays: user-optimized Other applications: user-optimized |
公司介紹：
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